Dietary riboflavin deficiency induces genomic instability of esophageal squamous cells that is associated with gut microbiota dysbiosis in rats
Type de document
journalArticle
Langue source
Anglais
Titre français
Titre anglais
Dietary riboflavin deficiency induces genomic instability of esophageal squamous cells that is associated with gut microbiota dysbiosis in rats
Auteur(s)
- PAN Feng
- XU Xin
- ZHANG Ling-Li
- LUO Hong-Jun
- CHEN Ye
- LONG Lin
- WANG Xuan
- ZHUANG Pei-Tong
- LI En-Min
- XU Li-Yan
Editeur(s)
Autre(s)
Id
TMWFDBY5
Version
2677
Date ajout
22 décembre 2020 14:19
Date modification
22 décembre 2020 14:19
Résumé anglais
Scope: Epidemiologic evidence suggests that riboflavin (RBF) deficiency is a specific nutritional predisposition for esophageal cancer. The aim of this study is to investigate the potential roles of gut microbiota in esophageal tumorigenesis caused by the RBF deficiency. Methods: Male F344 rats were subcutaneously injected with the chemical carcinogen N-nitrosomethylbenzylamine (NMBA, 0.35 mg kg−1). Rats were assigned to 4 groups, denoted as R6 (normal RBF, 6 mg kg−1), R6N (normal RBF combined with NMBA), R6N → R0N (normal RBF conversion to RBF-deficiency), and R0N → R6N (RBF-deficiency conversion to normal RBF). Bacterial communities were analyzed based on high-throughput 16S rRNA gene sequencing. Oxidative DNA damage and double-strand break markers were studied by immunohistochemistry. Results: The R6N → R0N diet enhanced the incidence of esophageal intraepithelial neoplasia (EIN, 40 weeks 66.7% vs. 25 weeks 16.7%, P < 0.05). RBF deficiency and replenishment modulated the gut microbiota composition. The gut microbiota (e.g. Caulobacteraceae, Sphingomonas and Bradyrhizobium) affected xenobiotic biodegradation and the genomic instability of the host. Furthermore, the RBF deficiency aggravated oxidative DNA damage and DNA double-strand breaks (immunohistochemistry) in the esophageal epithelium, whereas the RBF replenishment had the opposite effect (P < 0.05, respectively). Conclusions: RBF deficiency promotes NMBA-induced esophageal tumorigenesis, which is associated with gut microbiota-associated genomic instability, and offers new insights into the role of RBF deficiency in esophageal carcinogenesis.
Note
None
CRAW tags
- AB - Transversal
- FREDO alimentation animale
- GEO Chine
- riboflavin
WEB tags
Titre de la publication
Food & Function
Volume
11
Pages
10070-10083
Date caractères
2020-11-19
Date publication
19 novembre 2020
Doi
10.1039/D0FO01944E
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Issn
2042-650X
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