The mode of action of plant associated Burkholderia against grey mould disease in grapevine revealed through traits and genomic analyses
Type de document
journalArticle
Langue source
Anglais
Titre français
Titre anglais
The mode of action of plant associated Burkholderia against grey mould disease in grapevine revealed through traits and genomic analyses
Auteur(s)
- ESMAEEL Qassim
- JACQUARD Cédric
- SANCHEZ Lisa
- CLÉMENT Christophe
- AIT BARKA Essaid
Editeur(s)
Autre(s)
Id
QUZWVJRD
Version
2339
Date ajout
15 janvier 2021 10:05
Date modification
15 janvier 2021 10:05
Résumé anglais
Plant-associated Burkholderia spp. have been shown to offer a promising alternative method that may address concerns with ecological issue associated with pesticide overuse in agriculture. However to date, little work has studied the role of Burkholderia species as biocontrol agents for grapevine pathogens. To this end, two Burkholderia strains, BE17 and BE24 isolated from the maize rhizosphere in France, were investigated to determine their biocontrol potential and their ability to induce systemic resistance against grey mould disease in grapevine. Results showed the capacity of both strains to inhibit spore germination and mycelium growth of Botrytis cinerea. Experimental inoculation with BE17 and BE24 showed a significant protection of bacterized-plantlets against grey mould compared to the non-bacterized control. BE17 and BE24-bacterized plants accumulated more reactive oxygen species and an increased callose deposition was observed in leaves of bacterized plantlets compared to the control plantlets. In bacterized plants, gene expression analysis subsequent to B. cinerea challenge showed that strains BE17 and BE24 significantly increased the relative transcript level of pathogenesis-related (PR) proteins PR5 and PR10, two markers involved in the Salicylic acid (SA)-signaling pathway. Furthermore, in silico analysis of strains revealed the presence of genes involved in plant growth promotion and biocontrol highlighting the attractiveness of these strains for sustainable agricultural applications.
Note
None
CRAW tags
- AB - Utile à l'AB
- FREDO lutte
- FREDO santé végétale
- GEO France
- biocontrol agent
- viticulture
WEB tags
Titre de la publication
Scientific Reports
Volume
10
Pages
19393
Date caractères
2020-11-10
Date publication
10 novembre 2020
Doi
10.1038/s41598-020-76483-7
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Issn
2045-2322
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