Diversity and Activity of Free-Living Nitrogen-Fixing Bacteria and Total Bacteria in Organic and Conventionally Managed Soils
Type de document
journalArticle
Langue source
Anglais
Titre français
Titre anglais
Diversity and Activity of Free-Living Nitrogen-Fixing Bacteria and Total Bacteria in Organic and Conventionally Managed Soils
Auteur(s)
- ORR Caroline H.
- JAMES Angela
- LEIFERT Carlo
- COOPER Julia M.
- CUMMINGS Stephen P.
Editeur(s)
Autre(s)
Id
N4DT3M8V
Version
2770
Date ajout
6 janvier 2021 16:24
Date modification
6 janvier 2021 16:24
Résumé anglais
ABSTRACT
Agricultural soils are heterogeneous environments in which conditions affecting microbial growth and diversity fluctuate widely in space and time. In this study, the molecular ecology of the total bacterial and free-living nitrogen-fixing communities in soils from the Nafferton Factorial Systems Comparison (NFSC) study in northeast England were examined. The field experiment was factorial in design, with organic versus conventional crop rotation, crop protection, and fertility management factors. Soils were sampled on three dates (March, June, and September) in 2007. Total RNA was extracted from all soil samples and reverse transcribed. Denaturing gradient gel electrophoresis (DGGE) and quantitative PCR (qPCR) were used to analyze
nifH
and 16S rRNA genes in order to study free-living diazotrophs and the total bacterial community, respectively. Crop rotation was shown to have a significant effect on total bacterial diversity (and that of free-living N fixers) (
P
≤ 0.001). On all three dates,
nifH
activity was higher in the conventional crop rotation. In contrast, qPCR analysis of free-living N fixers indicated significantly higher levels of activity in conventionally fertilized plots in June (
P
= 0.0324) and in plots with organic crop protection in September (
P
= 0.0143). To our knowledge, the effects of organic and conventional farming systems on free-living diazotrophs have never been studied. An increased understanding of the impacts of management practices on free-living N fixers could allow modifications in soil management practices to optimize the activity of these organisms.
Note
None
CRAW tags
- AB - Modalité bio
- FREDO biologie et travail du sol
- GEO Royaume-Uni
- bacterial communities
- comparaison
WEB tags
Titre de la publication
Applied and Environmental Microbiology
Volume
77
Pages
911-919
Date caractères
2011-02-01
Date publication
1 février 2011
Doi
10.1128/AEM.01250-10
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Issn
0099-2240, 1098-5336
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