Application of LC-HRMS identified marker peptides in an LC-MS/MS method for detection and quantification of heat-resistant proteolytic activity in raw milk
Type de document
journalArticle
Langue source
Anglais
Titre français
Titre anglais
Application of LC-HRMS identified marker peptides in an LC-MS/MS method for detection and quantification of heat-resistant proteolytic activity in raw milk
Auteur(s)
- VERHEGGHE Marijke
- BLOCK Jan De
- HEYNDRICKX Marc
- COILLIE Els Van
- POUCKE Christof Van
- DUQUENNE Barbara
Editeur(s)
Autre(s)
Id
MYATNB9E
Version
3121
Date ajout
15 janvier 2021 10:05
Date modification
15 janvier 2021 10:05
Résumé anglais
For the dairy industry, it would be useful to be able to determine the heat-resistant proteolytic activity of the raw milk upon arrival at the plant, preferably before processing (within 24 h of arrival). This would allow producers to choose the most optimal processing of the raw milk and hence its fate, together with a more accurate prediction of shelf-life. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the detection and quantification of Pseudomonas-derived heat-resistant proteolytic activity in raw milk based on three new marker peptides, identified using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS), was developed. This method involved a sample preparation where existing peptides are removed from the milk sample, after which the heat-resistant protease-containing fraction is mixed with a casein substrate. After incubation, the increase of the peptide markers is determined using an LC-MS/MS method. The limit of detection (LOD) and limit of quantification (LOQ) of this method were determined using synthetically produced peptides (with an average LOD between 0.1 and 5 ng/mL and an average LOQ between 0.1 and 10 ng/mL).
Note
None
CRAW tags
- AB - Transversal
- FREDO qualité des produits
- FREDO technologie et innovation
- GEO Belgique
- GEO Flandre
- lait
WEB tags
- LC-HRMS identification
- LC-MS/MS detection
- marker peptides
- proteolytic activity
- pseudomonas
- raw milk
Titre de la publication
International Journal of Dairy Technology
Volume
n/a
Date caractères
2020
Date publication
1 janvier 2020
Doi
https://doi.org/10.1111/1471-0307.12754
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Issn
1471-0307
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