Pesticide risk assessment at the molecular level using honey bee cytochrome P450 enzymes: A complementary approach
Type de document
journalArticle
Langue source
Anglais
Titre français
Titre anglais
Pesticide risk assessment at the molecular level using honey bee cytochrome P450 enzymes: A complementary approach
Auteur(s)
- HAAS Julian
- NAUEN Ralf
Editeur(s)
Autre(s)
Id
52W39WQN
Version
2786
Date ajout
15 janvier 2021 23:33
Date modification
15 janvier 2021 23:33
Résumé anglais
Honey bee (Apis mellifera) first-tier pesticide risk assessment is largely based on standardized laboratory toxicity bioassays after both acute and chronic exposure. Recent research on honey bee cytochrome P450 monooxygenases (P450s) uncovered CYP9Q3 as the molecular determinant mediating neonicotinoid insecticide selectivity and explaining why certain neonicotinoids such as thiacloprid show > 1000-fold lower acute toxicity than others (e.g. imidacloprid). Here this knowledge is leveraged for mechanistic risk assessment at the molecular level using a fluorescence-based high-throughput in vitro assay, predicting the interaction of diverse pesticidal chemotypes, including azole fungicides, with recombinantly expressed honey bee CYP9Q enzymes, known to metabolize thiacloprid, acetamiprid and tau-fluvalinate. Some azole fungicides were shown to be synergistic in combination with certain insecticides, including neonicotinoids and pyrethroids, whereas others such as prothioconazole were not. We demonstrate that biochemical CYP9Q2/CYP9Q3 inhibition data of azoles revealed a striking correlation with their synergistic potential at the organismal level, and even allow to explain combined toxicity effects observed for tank mixtures under field conditions. Our novel toxicogenomics-based approach is designed to complement existing methods for pesticide risk assessment with unprecedented screening capacity, by utilizing honey bee P450 enzymes known to confer pesticide selectivity, in order to biochemically address issues of ecotoxicological concern.
Note
None
CRAW tags
- AB - Non-spécifique
- FREDO environnement
- GEO Allemagne
- pesticide
WEB tags
- apis mellifera
- molecular ecotoxicology
- pesticide mixtures
- pollinator safety
- synergism
- toxicogenomics
Titre de la publication
Environment International
Volume
147
Pages
106372
Date caractères
February 1, 2021
Date publication
1 février 2021
Doi
10.1016/j.envint.2020.106372
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Issn
0160-4120
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